Figure 1

Summary of expression of transcripts for isoforms of epithelial Ca²⁺ channels (TRPV5 and TRPV6), calbindin, NCX, PMCA and VDR with and without exposure to 1,25- (OH) ₂ vitamin D ₃ . Expression levels given as ratio of specific gene to 18S in primary cultures of semicircular canal duct (SCCD), native SCCD, native lateral wall (LW) and native stria vascularis (SV). All native tissues were incubated overnight after microdissection in order to reduce extracellular cross-contamination from RNA released from adjacent tissues (see Fig 1B). Open bars; control, hatched bars; 1,25-(OH)₂vitamin D₃ treated, Significant up- or down-regulation is indicated; *, P < 0.05; ND, not detected. For comparison, data in the left section are primary culture of SCCD, reproduced from [5].