Figure 2

MIP and DH31 expression in Midgut Junction DH31 expressing cells and junction region. In A-F, midgut junction regions are oriented with the anterior in the upper right hand corner and the acidic region in the lower left hand side. G-I, the luminal/apical portion of the enteroendocrine cell is on the left and the basal portion of the cell is on the right. The size bars in A-F are 50 um the size bar for G-I is 20 um. A, D, G are DJ752 Gal4/UAS GFP-CD8 reporter gene expression. There are 4 Midgut Junction DH31 expressing cells in (A) and (D). (B) is expression of Allostatin B/MIP in the junction region, where there are eight cells expressing Allostatin B/MIP. C) is the merged image of A and B, note the distinct red and green cells that demonstrate two distinct groups of cells. (E) is the expression of DH31 peptide. (F) is the merged image of (D) and (E). Note that the DH31 expressing cells co-express GFP-CD8 and appear yellow. (G) through (I) are a close-up of the Midgut Junction DH31 expressing cells expressing the DH31 peptide hormone. In (G) the Midgut Junction DH31 expressing cells express the membrane bound GFP-CD8 outlining the cells basal body and luminal apical projection (arrow). (H) All of the DH31 peptide is localized to the basal portion of the cell but not in the apical projection. This is more apparent in (I), with the apical projection shown in green without co-localization of DH31. However, in the basal portion of the cell GFP and DH31 co-localize (yellow).