Figure 5
From: Western diet enhances hepatic inflammation in mice exposed to cecal ligation and puncture
Chemokine and TLR-4 expression in palmitic acid-treated C3A hepatocytes. C3A hepatocytes were cultured in medium containing lipopolysaccharide (LPS) (500 pg/ml), palmitic acid (PA; 100 mM) or the combination of PA and LPS for 24h. Expression of (A) IL-8 and (B) TLR-4 was assessed using pre-developed assays for real-time PCR and values were calculated using a comparative CT method according to the manufacturer instructions (Applied Biosystems). Data are presented as mean SEM of 4 experiments. Statistical analysis was performed using two-way ANOVA and Tukey's multiple comparisons test. *p < 0.05 compared to vehicle control cultures; #p < 0.05 compared to PA alone.