Gene expression and activity of digestive proteases in Daphnia: effects of cyanobacterial protease inhibitors

BMC Physiology

Table 2 proteases of D. magna

Protease	Accession number	Primer forward	Primer reverse	Apparent MW [kDa]	True MW [kDa]
T152	DMC00580	5'-TGGATGCTCCATTGGACTTGA-3'	5'-CGGAAACGGTGACGATGATTC-3'	32-34	25.3
T208	DMC05983	5'-TGCGTTAGAGGAGTTGACGCT-3'	5'-TGAAGCTGACAACACCACGGT-3'	24-25
CT383	DMC00886	5'-TTGGCACCTTCCACCGAAT-3'	5'-TCATCAGGACTGGAGAAACGC-3'	20	23.98
CT448	DMC01045	5'-TCATCAACGGAGCTGAGGCTA-3'	5'-AGAACCCACTGGTCGGAAATC-3'	21-22	26.4
T610	DMC00622			75	24.1
CT802	DMC00275	5'-TCAGATTGCCCAACCCCAT-3'	5'-TCCGCTGATGTGGAGCATATC-3'	17-19	24.7

Delineated are the names and the accession numbers of the proteases of D. magna from the EST-database search and the established forward and reverse primers for the QPCR analyses (no primers for T610 were established). Apparent molecular weights (MW) resulted from the comparison of the molecular weight marker with the visible protease bands in the SDS-PAGE, whereas true MW resulted from the translation of the sequences (start- to stop-codon) of the EST-database (http://www.nematodes.org/NeglectedGenomes/ARTHROPODA/Crustacea.html; [34]). No complete sequence was available for T208. (CT = chymotrypsin, T = trypsin).

ISSN: 1472-6793