Figure 2

Expression of MsSCP-x and MsSCP-2. (A) The semi-quantitative RT-PCR shows that mRNA levels from different tissues at different developmental stages of M. sexta. Lane A: Day 1-4^th instar; lane B: Day 3-4^th instar; lane C: Day 1-5^th instar; lane D: Day 4-5^th instar; lane E: Day 1-wandering stage; lane F: Day 1-pupal stage. The Manduca rpS3 gene was used as internal standard for the RT-PCR. Only 30 cycles of PCR were performed to avoid saturating the signals. (B). Proteins from different tissues of Day 3-4^th instar M. sexta larva were loaded 20 μg per lane except lane 8 that only had 10 ng purified recombinant MsSCP-2. Protein was analyzed on 4-20% gradient SDS PAGE. Lane 1: protein size marker; Lane 2: hemolymph; Lane 3: midgut; Lane 4: hindgut; Lane 5: fat body; Lane 6: muscle; Lane 7: epidermis; Lane 8: recombinant MsSCP-2.