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Figure 4 | BMC Physiology

Figure 4

From: Sterol carrier protein-x gene and effects of sterol carrier protein-2 inhibitors on lipid uptake in Manduca sexta

Figure 4

NBD-cholesterol and SCPIs competition binding assays to MsSCP-2. Assays for NBD-cholesterol and sterol carrier protein-2 inhibitors (SCPIs; A: SCPI-1; B: SCPI-2) competitive binding to MsSCP-2. The background NBD-cholesterol fluorescence (NBD-cholesterol alone in the reaction buffer) was deducted from each assay. Shown are net changes in NBD-cholesterol fluorescence in intensity (RFU = Relative fluorescence unit) in the presence of increasing concentrations of each SCPI. GST and AeSCP-2 were used as negative and positive controls, respectively. The data were processed using PRISM4.0 software.

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BMC Physiology

ISSN: 1472-6793

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