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Figure 4 | BMC Physiology

Figure 4

From: The ΔF508-CFTR mutation inhibits wild-type CFTR processing and function when co-expressed in human airway epithelia and in mouse nasal mucosa

Figure 4

Stable Transfection of ΔF508-CFTR into CALU-3 non-CF epithelial cells alters the processing and function of endogenous WT-CFTR. A. CFTR was IP’d from parental and stably transfected CALU-3 cells. The samples were then phosphorylated and resolved as described previously. B. Parental and stably transfected CALU-3 cells were seeded on 6.5 mm Vitrogen coated permeable filter supports. The CALU-3 monolayers were then allowed to reach a transepithelial resistance (RTE) of 2,000 Ω·cm2 or above prior to experimentation. ISC is then measured in response to 10 μM amiloride, 20 μM forskolin and 50 μM genistein added apically via an Ussing chamber. Typical traces are shown. C. Summary analysis of parental and stably transfected CALU-3 cells ISC response to agonists.

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BMC Physiology

ISSN: 1472-6793

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