Figure 2

Time courses for the effects of cycloheximide and actinomycin D. Young rats were fasted overnight and were then injected with cycloheximide (CHX, panels A and D-F) or ActD (panels B, C and G-I) starting at 8:00 h. Groups of rats (n=5) were sacrificed at the indicated times over a 4 or 6 hour period. Rats for the time 0 groups were not injected and were sacrificed within one hour from the start of the experiment. Epididymal adipose tissue was taken for analyses. (A) LPL activity (●) and mass (■) after CHX. (B) LPL activity (●) and mass (■) after ActD (C) mRNA levels for ANGPTL4 (■), GPIHBP1 (▲), LPL (●) and LMF1 (▼). mRNA was calculated relative to 18S mRNA. The value at time 0 was set to 1 and values at following time points calculated relative to this. (D-I) Western blots of adipose tissue as labeled in the figure. The antibodies to C-terminal ANGPTL4 used in panels E and H recognize both full-length ANGPTL4 and the fibrinogen-like C-terminal domain. The antibodies to the N-terminal domain of ANGPTL4, used in panels F and I, identifies the full-length protein but do not see the C-terminal domain.