Figure 8

Effects of age and/or obesity. Groups of rats of different ages (5 weeks, 26 weeks and 52 weeks) were either fed ad lib (solid bars) or fasted overnight for 16 h (open bars). At 8:00 h groups of fed and fasted rats of the indicated age were sacrificed (n=5 per group). The epididymal fat body was cut out and weighed. Blood was also taken for analyses of (A) glucose, (B) NEFA and (C) Insulin. mRNA levels for ANGPTL4 (D) and GPIHBP1 (E) and are expressed relative to 18S RNA. (F) LPL activity expressed per g wet weight of tissue. Statistical comparisons are made between fed and fasted animals. The numbers above some of the bars represent the relative change from the fasted to the fed state.