Figure 7

Islet LPL was mainly intracellular in WT mice but absent in ob/ob mice. Islets from WT and ob/ob mice stained for LPL and endothelium (with anti-CD31). (A) LPL in an islet from a WT mouse. (B) Staining for CD31 and (C) a merge showing co-localization between LPL and CD31. (D) Pancreatic tissue from an ob/ob mouse, LPL staining was only visible in the periphery of the islet (similar to the pattern for alpha cells). (E) Anti-CD31 demonstrates small vessels in the islet and (F) a merge between D and E showing that most of the LPL reaction was separate from the staining of vessels.