Figure 5

Representative photomicrographs of bladder whole mounts showing ChAT-positive nerve fibers, sensory nerves (TRPV1), and blood vessels (CD31). Representative photomicrographs taken from bladders isolated from ChAT mice and prepared as whole mounts that underwent blunt dissection to separate the lamina propria from the detrusor. In the lamina propria (D) the ChAT and TRPV1 fibers are usually associated (yellow arrow) but sometimes take separate paths (red and green arrows). In the detrusor (H) ChAt-positive fibers dominate although the ChAT and TRPV1 fibers around blood vessels are usually associated (yellow arrow). High magnification microphotographs (I-L) indicate that the two type of fibers run adjacent to each other (L).