Figure 2

IL-1β response to acute stressor exposure in visceral and subcutaneous WAT. Effect of acute stressor exposure on the IL-1β protein content of visceral (omental) and subcutaneous (inguinal) WAT in healthy, non-obese rats. 21 week old male F344 rats were exposed to 50 inescapable 1.5 mA tail shocks (stress, n = 24) or remained in their home cages (no stress, n = 26). Rats were sacrificed immediately following stressor exposure. Approximately 0.3 g of omental and subcutaneous WAT depots were harvested, spot frozen in liquid nitrogen and processed via homogenization in a RIPA lysis buffer. IL-1β protein analysis (pg IL-1β per mg total WAT protein) was done via ELISA (R&D Systems Minneapolis, MN). Effect of depot on IL-1β concentration F(1,46) = 15.751, **P < 0.001. Effect of stress on IL-1β content: F(1,46) = 28.461, ***P < 0.0001. Depot by stress interaction: F(3,44) = 13.312, ##P < 0.001. Values represent group means + standard error of measurement. All experiment protocols were approved by the Animal Care and Use Committee of the University of Colorado at Boulder.