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Table 2 List of primers used for the qPCR reactions

From: Disease resistance is related to inherent swimming performance in Atlantic salmon

Genes Short name Sequence 5' to 3' Accession
radical S-adenosyl methionine domain-containing protein 2 1 Rsad2 F-GTACCGCAGATGCACAACAC AF076620
   R-TTGACACTGCTTGGAGTTGC  
interferon inducible protein Gig1 1 Gig1 F-GGCAACCTGAATCCAGAAGA DW569595
   R-GTCTGGACGCAGACTGATGA  
VHSV-inducible protein 1 Vhsv2 F-GGTGAAGACCTGGACCTGAA BT072288
   R-TGACCCCTGTTGACCTTCTC  
interferon-induced protein with tetratricopeptide repeats 5 1 Ifit5 F-CAGAGAGGTGCCAGGCTAAC BT046021
   R-TGCACATTGACTCTCCTTGG  
interferon induced with helicase C domain 1 1 Mda5 F-CAGAGGTGGGGTTCAATGAT NM001195179
   R-AGCTCGCTCCACTTGTTGAT  
DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide RIG-I 1 Rig-I F-GACGGTCAGCAGGGTGTACT DY714827
   R-CCCGTGTCCTAACGAACAGT  
barrier-to-autointegration factor 1 Baf F-ACAGACCCCTCATCATCCTG BT049316
   R-CGGTGCTTTTGAGAAGTGGT  
signal transducer and activator of transcription 1 isoform alpha 1 Stat1a F-CGGTGGAGCCCTACACTAAG CB513054
   R-GGGATCCTGGGGTAGAGGTA  
interferon-inducible protein Gig2-like 1 Gig2 F-GATGTTTCATGGCTGCTCAA BT044026
   R-CTTTTCGGATGTCCCGACTA  
B-type natriuretic peptide 2 BNP F-TCGACAAATCCGCAATAAGA CK883650
   R-TTGAGCCAATTCGGTCTAGC  
putative collagen alpha 1 2 put_Coll-a1 F-AACCCTGAACCCCTCAGTCT CA038317
   R-TGGTCCTACCGTCTGGTTTC  
leukocyte elastase inhibitor 2 LEI F-TCTCAGATGGCAAAGGCTCT BT045959
   R-GTTGGCCAGTTTCAGGATGT  
elongation factor 1a 3 EF1α F-CACCACGGGCCATCTGATCTACAA BT072490
   R-TCAGCAGCCTCCTTCTCGAACTTC  
18S rRNA 3 18S F-GCCCTATCAACTTTCGATGGTAC AJ427629
   R-TTTGGATGTGGTAGCCGTTTCTC  
infectious pancreatic necrosis virus_polyprotein 4 IPNV F-CCGACCGAGAACAT AJ877117
   R-TGACAGCTTGACCCTGGTGAT  
  1. 1 Virus-responsive genes (Figure 4).
  2. 2 Genes used for microarray validation.
  3. 3 Reference genes used for normalization.
  4. 4 IPNV gene used for relative virus quantification (described in Results section).