Figure 2
Antibiotics diminished leukocyte and platelet adherence and rolling induced by feeding dietary oils in jejunum venules of WT C57/BL6J mice. Mice were pre-treated with saline or antibiotics (450 mg/kg/day polymyxin B and 150 mg/kg/day neomycin) by gavage for 7 days. After 7 days, olive oil or corn oil was administered and mice subjected to intravital microscopy at ‘peak’ time point respectively (2.0 h after olive oil gavage or 1.5 h after corn oil gavage). Antibiotics significantly diminished leukocyte and platelet adherence to post-capillary venules 2 h following gavage with olive oil (*P < 0.05 vs. Saline control. #P < 0.05 vs. Olive 2.0 h group) [A] or 1.5 h after gavage with corn oil (*P < 0.05 vs. Saline control. #P < 0.05 vs. Corn 1.5 h group) [B]. Antibiotics also significantly reduced leukocyte and platelet rolling in post-capillary venules induced by gavage with olive oil for 2 h (*P < 0.05 vs. Saline control. #P < 0.05 vs. Olive 2.0 h group) [C] or with corn oil for 1.5 h (*P < 0.05 vs. Saline control. #P < 0.05 vs. Corn 1.5 h group) [D].