Taurocholic acid (TCA) uptake and cellular localization of wild-type and Pro107mutant Slc10a2 in stably transfected LLC-PK1 cells. A, Untransfected or stably transfected LLC-PK1 cells were incubated with 0.1 μM [G-3H]TCA (44.03 GBq/mmol; Perkin-Elmer) in the presence of 100 mM NaCl (shaded columns) or 100 mM choline chloride (open columns) at 37°C for 1 min. Each column and error bar represents the mean and standard error (SE) of 3 independent experiments, respectively. B, Whole cell lysates (left panel) and plasma membrane fractions of untransfected or stably transfected LLC-PK1 cells were divided into 2 equal parts and subjected to 12.5% SDS-polyacrylamide gel electrophoresis (PAGE). Western blotting was performed using anti-green fluorescent protein (GFP) and anti-calnexin (endoplasmic reticulum marker) antibodies. Filled and open arrowheads indicate fully glycosylated and unglycosylated forms, respectively. Numbers denote molecular weight markers.