Table 2 Partial sequences of KCNQ4 obtained from PCR products
From: Kcnq 1-5 (Kv7.1-5) potassium channel expression in the adult zebrafish
KCNQ4 (a) primers | |||||
|---|---|---|---|---|---|
5′- | |||||
NNNNNNNNNN | CGGGCAGGGC | AAGAAGNNCT | CCCAGAAGGG | CAAAACAAGC | TGCTAAAAGA |
CGACCTTGCC | AGGTGTGTGG | AGTCTTGTCA | CCGTAGCCGA | TCGTAGTCAG | GGTTATCGTC |
CCCCACCAGA | GGGAGTCTGC | ATAA | Â | Â | Â |
-3′ (144 bp) | |||||
KCNQ4 (b) primers | |||||
5′- | |||||
NNNNNNTNNG | ATGATATTCT | CGCTCCTCTC | AGGCCATCCG | GAGCAAGGCT | TCTCCTTTA |
CTCCAGGTAA | CGTGCGGTGT | TCACCCAGCA | CTGAGAACGT | CCCAGAAGCC | ACCAGCCCTG |
GGAAAGTGCA | GAAAAGCTGG | AGCTTCAATG | ACCGGACACG | TTTTCGCACA | TCTCTGCGCC |
TCAAACCACG | ACCCGCTGCA | GACATGGAGG | GAGTCGGAGA | AGAGCACACT | GAGGACAAAT |
CTTACTGTGA | CGTGGCCATG | GAGGATGTGA | TTCCCGCAGT | GAAGACCCTG | ATTCGAGCGG |
TTCGGATCCT | GAAGTTCCTG | GTGGCCAAGA | GGAAGTTTAA | AGANCCCCTT | GCCGGCCGAA |
NNG | Â | Â | Â | Â | Â |
-3′(363 bp) | |||||
KCNQ4 (c) primers | |||||
5′- | |||||
CNNNNNNTNG | GATGATNTTC | TCGCTCCTCT | CAGGCCATCC | GGAGCAAGGC | TTCTCCTTTA |
CCTCCAGGTA | ACGTGCGGTG | TTCACCCAGC | ACTGAGAACG | TCCCAGAAGC | CACCAGCCCT |
GGGAAAGTGC | AGAAAAGCTG | GAGCTTCAAT | GACCGGACAC | GTTTTCGCAC | ATCTCTGCGC |
CTCAAACCAC | GACCCGCTGC | AGACATGGAG | GGAGTCGGAG | AAGAGCACAC | TGAGGACAAA |
TCTTACTGTG | ACGTGGCCAT | GGAGGATGTG | ATTCCCGCAG | TGAAGACCCT | GATTCGAGCG |
GTTCGGATCC | TGAAGTTCCT | GGTGGCCAAG | AGGAAGTTTA | AAGAAACCCT | GCGGCCGA |
-3′ (358 bp) | |||||