Figure 2

Blockade of MTX-induced Ca²⁺ influx by U73343 is partially reversible. Prior to fluorescence recording, fura-2 loaded BAECs were equilibrated with HBS containing either 5 μM U73343 or 0.1% DMSO for 5 min. The cells were washed to remove the drug which required ~5 min. The cells were resuspended in the absence of U73343, immediately placed in a cuvette, and the fluorescence recorded. Addition of 0.3 nM MTX at 100 sec to the DMSO-treated cells (black trace) resulted in a typical MTX response. Addition of MTX to the U73343 pretreated cells at 100 (red), 200 (green), 300 (yellow), 400 (blue) or 500 sec (purple) are shown superimposed. Results shown are representative of 3 independent experiments.