Figure 4

U73343 rescues the cells from MTX-induce lytic cell death. MTX-induced change in [Ca²⁺]_i (upper panel) or EB uptake (lower panel) were assayed in BAECs. MTX and U73343 were added at the concentrations and time indicated. LDH release was measured as described in Materials and Methods and plotted in the lower panel using color matched symbols (see inset). All experiments were performed in a paired fashion on the same batch of BAECs. Results shown are representative of 4 independent experiments.