Figure 4

Inhibition of soluble guanylate cyclase prevents the AQP1 conductance response to SNP. Wild type AQP1-expressing and control oocytes were incubated three hours in ND96 saline with or without an inhibitor of soluble guanylate cyclase (1H-[1, 2, 4]oxadiazolo [4,3-a]quinoxalin-1-one, ODQ, 10 μM) prior to electrophysiological analysis of the response to SNP (doses indicated in mM on horizontal axis). Data are mean ± SEM; n = 3 or 4 oocytes per group. An asterisk (*) indicates values that are significantly different (p < 0.04) from control; the pound sign (#) indicates values that are significantly different (p < 0.04) from wild type AQP1 (unpaired two-tailed Student's t test).