Figure 2
From: Heat induced HSP20 phosphorylation without increased cyclic nucleotide levels in swine carotid media
Recovery after heat treatment. Swine carotid artery tissues were exposed to 44.5°C for 4 hours and then either 1) stimulated with 10 μM histamine for 10 min before freezing (0 recovery time) or returned to 37°C for various times and then stimulated with 10 μM histamine for 10 min before freezing (positive recovery times). A unheated control set of tissues was stimulated with 10 μM histamine for 10 min and frozen without 44.5°C heat treatment (labeled "before"). Histamine induced ser16-HSP20 phosphorylation (mol Pi at ser 16 / mol HSP20) is shown in the top panel, putative PKC-HSP20 phosphorylation (mol Pi at the PKC site / mol HSP20) in the center panel, and force in the bottom panel (n = 4–6). Force was significantly reduced in all heat treated tissues regardless of recovery time compared to the unheated control. Ser16-HSP20 phosphorylation was significantly higher in all heat treated tissues regardless of recovery time compared to unheated control.