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Table 3 Brief anesthesia with carbon dioxide before sacrifice does not increase plasma corticostrone.

From: Role of glucocorticoids in mediating effects of fasting and diabetes on hypothalamic gene expression

Method of Sacrifice

  

Treatment groups

n

Corticosterone (ng/ml)

Mice with CO2

8

85.25 ± 12.47

Mice without CO2

5

96.4 ± 23.22

Rat with CO2

5

106 ± 30.79

Rat without CO2

5

203 ± 79.12

  1. Mice or rats were either sacrificed with carbon dioxide as described in the Methods section, or decapitated immediately after removing from cage. Data are expressed as mean ± SEM. By either paired or unpaired t-test, there was no effect of method of sacrifice (p > 0.05).