Figure 1

HCO ₃ ^--induced up-regulation of sAC mRNA expression. RT-competitive PCR was performed using specific sAC primers and GAPDH internal standard primers from cultured BCECs. A: RT-competitive PCR of sAC mRNA from cultured BCECs exposed to different [HCO₃^-]. From left to right: M: Marker; NC: negative control (without cDNA); [HCO₃^-]: 0, 5, 10, 20, 40, 60 and 80 (mM). B: summary of the ratios of band densities (sAC/GAPDH) from A. Error bar indicates ± SE (n = 3). C: RT-competitive PCR of sAC mRNA from cultured BCECs exposed to 40 mM HCO₃^- with different incubation time. From left to right: M: Marker; NC: negative control (without cDNA); Time: 0, 3, 6, 12, 24, 48 (hours). D: summary of the ratios of band densities (sAC/GAPDH) from C. Error bar indicates ± SE (n = 3).