Table 1 Primers used for AQPs and solute transporters expression
Gene | Primers (forward; reverse) | Amplicon size, bp | cDNA sequence location |
|---|---|---|---|
AQP0 | 5'-acggctcaagagtgtttctga-3' | 189 | 669-689 |
5'-tccccacagtctctttcttcat-3' | 857-836 | ||
AQP8 | 5'-aagaccatgctgctaattcc-3' | 275 | 423-442 |
5'-tccacaatgacagagaaacc-3' | 697-678 | ||
AQP9 | 5'-tgttgtcattagcctcctgatc-3' | 356 | 736-757 |
5'-tgaagaaagaactggatgaacg-3' | 1091-1070 | ||
MRP2 | 5'-ctggttggaaacttggtcgt-3' | 172 | 3719-3739 |
5'-caactgccacaatgttggtc-3' | 3890-3870 | ||
BSEP | 5'-cactggccttctggtatggt-3' | 225 | 1275-1294 |
5'-gcttgtagccgtctcctgac-3' | 1499-1479 | ||
AE2 | 5'-tctcgttctgcaagagcaacc-3' | 270 | 2527-2547 |
5'-ttgttactgctgctgtctgcc-3' | 2797-2777 |