Figure 3
Tumor Necrosis Factor-α and Interferon-γ Synergize to Elevate Flux in MDCK cells. MDCK cells were treated with increasing dose of TNFα/IFNγ for up to 72 hours; TER and [3H]-mannitol flux were determined. Panel A reports the mean TER when cells were exposed to the following conditions; media only control (
), TNFα/IFNγ, 3/6 ng/ml (
), TNFα/IFNγ, 10/20 ng/ml (
), and TNFα/IFNγ, 30/60 ng/ml (
). Panel B reports the mean [3H]-mannitol flux following 72 hour incubation with the indicated treatments. Flux is presented as the percent of apical [3H]-mannitol recovered in the basolateral chamber following 120 min. incubation. Error bars represent the SE, n = 6. A one-way analysis of variance (ANOVA) was performed, multiple comparisons between control and treatments were determined with the Bonferroni post test. **Indicates statistical difference (P < 0.001) to control.