Figure 7

Aberrant Localization of Tight Junction Proteins and Cytoskeletal Reorganization induced by Proinflammatory Cytokines in MDCK Cells. Immunofluorescence microscopy was used to capture digital images of MDCK cells grown to confluency on glass coverslips. Images A, D, G, J and M show control occludin, claudin-1, claudin-2, claudin-3 and actin staining respectively. Images B, E, H, K and N demonstrate occludin, claudin-1, claudin-2, claudin-3 and actin staining following twenty-four treatment with TNFα/IFNγ (10 and 20 ng/ml). Cells were treated TNFα/IFNγ for a 24 hour interval in the presence the ERK1/2 inhibitor U0126, 1 μM, representative images C, F, I, L and O were stained for occludin, claudin-1, claudin-2, claudin-3 and actin respectively. All images were captured on a Nikon 2000E microscope using a 60X oil-immersion lens, the calibration bar represents 25 μm.