Figure 4
From: Protein expression of G-protein inwardly rectifying potassium channels (GIRK) in breast cancer cells
Decreases in GIRK1, GIRK 2, and GIRK4 protein levels in MDA-MB-453 cells after treatment with ethanol (0.12%) pre-treated with serum free media for 16 hours. Top panel: GIRK1 protein expression was decreased by 5–30 minute ethanol treatment. GIRK1 expression was changed at both MWs. Serum-free media (used in previous research for 16 hours prior to starting experiment) inhibited GIRK1 protein expression and was not used after these ethanol experiments. Second panel: GIRK2 protein expression was decreased by 5–30 minute ethanol treatments. Serum-free media also inhibited GIRK2 protein expression. Third panel: GIRK4 protein expression was decreased by 5–30 minute ethanol treatment. Serum-free media also decreased GIRK4 protein expression. Bottom panel: Membranes were additionally probed with an antibody for actin to ensure equal loading of protein between samples. This is a representative gel of two separate experiments. Graphs indicate densitometry of bands, and data are shown as a ratio of GIRK/actin.