Dose-dependent increase in the percent ethidium-labeled proximal tubule epithelial cells in the renal cortex following treatment with Hg2+. Digital images from three fields per microscope slide with a total of four slides from each animal were quantified for necrosis analysis. Each animal represents one n value or replicate, in each treatment group. The values for each data point represent the mean ± SE for a total of n ≥ 6 for each treatment group. Asterisk (*) indicates significant differences from 0.9% NaCl vehicle control treatment group (one-way ANOVA, p < 0.001, post-hoc Tukey's Test).