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Figure 6 | BMC Physiology

Figure 6

From: Inflammatory cytokines induce MAdCAM-1 in murine hepatic endothelial cells and mediate alpha-4 beta-7 integrin dependent lymphocyte endothelial adhesion In Vitro

Figure 6

TNF-α induces MAdCAM-1 dependent adhesion of TK-1 lymphocytes to HEC. Compared to controls which exhibited 57.68 ± 3.77% of the maximum lymphocyte adhesion, 20 ng/ml TNF-α (24 h), induced a significant increase in TK-1 cell adhesion (set as maximal '100%' adhesion). The increased adhesion induced by TNF-α was significantly reduced to 84.8 ± 3.99% of maximal adhesion by pre-/co-treatment of HEC with the blocking mouse MAdCAM-1 Ab 'MECA-367'(10 ug/ml). The binding but not blocking MAdCAM-1 antibody MECA-89 did not significantly reduce TNF-α induced adhesion (98 ± 5.25% maximal adhesion). Each value represents the mean ± s.d.; each group (n = 6).

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BMC Physiology

ISSN: 1472-6793

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