Table 1 List of bioassays used for characterization in the initial screen and result summary

Cardiovascular, renal and bladder function

Bladder function (F)

NS

Blood pressure/heart rate (M)

↑(BP)*

Urine chemistry panel (F)

NS

CNS and Behavior

Irwin test (M,F)

↓***

Cross-therapeutic assays

Automated blood chemistry panel (M)

NS

Body weight measurements (M,F)

NS

Corticosterone (M)

↑*

Immunology and Inflammation

DTH (M)

NS

FACS analysis (M)

↓*

LPS challenge (M)

NS

Monocyte infiltration (F)

↑*

Pulmonary inflammation (F)

NS

Wound healing (F)

NS

Metabolism and Body Composition

DEXA (F)

↓*

Excised bone DEXA (F)

↓**

Food intake (M,F)

NS

Food intake on HFD (M)

↑***

Leptin (M,F)

↓**

Metabolic response to HFD (M)

↑**

Physical response to HFD (M)

↓***

Selected muscle weight (F)

↓**

Pain

Formalin pain assay (M)

NS

Hot-plate assay (F)

NS

Sexual health

Histopathology of reproductive system (M)

NS

Induced erection test (M)

↓*

Male contact sexual behavior (M)

↓***

Male fertility (M)

↓***

Testosterone (M)

NS