Hepatic steatosis in n-3 fatty acid depleted mice: focus on metabolic alterations related to tissue fatty acid composition

Table 1 Sequences for the primers used in real-time quantitative PCR

	GenBank accession no.	Forward primer (5' to 3')	Reverse primer (5' to 3')
PGC1α	NM_008904.1	AGCCGTGACCACTGACAACGAG	GCTGCATGGTTCTGAGTGCTAAG
CPT1a	NM_013495.1	AGACCGTGAGGAACTCAAACCTAT	TGAAGAGTCGCTCCCACT
PPARα	NM_011144.3	CAACGGCGTCGAAGACAAA	TGACGGTCTCCACGGACAT
PPARγ	NM_011146.2	CTGCTCAAGTATGGTGTCCATGA	TGAGATGAGGACTCCATCTTTATTCA
FAS	NM_007988.3	TTCCAAGACGAAAATGATGC	AATTGTGGGATCAGGAGAGC
SREBP-1c	NM_011480.2	GATCAAAGAGGAGCCAGTGC	TAGATGGTGGCTGCTGAGTG
NOX	NM_007807.2	TTGGGTCAGCACTGGCTCTG	TGGCGGTGTGCAGTGCTATC
MTTP	NM_008642.1	ATGATCCTCTTGGCAGTGCTT	TGAGAGGCCAGTTGTGTGAC
DGAT2	NM_026384.3	ACTCTGGAGGTTGGCACCAT	GGGTGTGGCTCAGGAGGAT
GPAT1	NM_008149.3	GTCCTGCGCTATCATGTCCA	GGATTCCCTGCCTGTGTCTG
G6Pase	NM_008061.3	AGGAAGGATGGAGGAAGGAA	TGGAACCAGATGGGAAAGAG
PEPCK	NM_011044.2	ACCTCCTGGAAGAACAAGGA	CTCATGGCTGCTCCTACAAA
L-FABP	NM_017399.2	ACCTCATCCAGAAAGGGAAGG	ACAATGTCGCCCAATGTCATG

PGC1α; peroxisome proliferator-activated receptor gamma coactivator, CPT1; carnitine palmitoyl transferase 1, PPAR; peroxisome proliferator-activated receptor, FAS; fatty acid synthase, SREBP-1c; sterol-regulatory-element-binding protein-1c, NOX; NADPH oxidase, MTTP; microsomal triglycerides transfert protein, DGAT2; Diacylglycerol acyl transferase 2, GPAT1; Glycerol phosphate acyl transferase 1, G6Pase; glucose 6-phosphatase, PEPCK; phosphoenolpyruvate carboxykinase, L-FABP; liver fatty acid binding protein

ISSN: 1472-6793