Figure 4

Determination of knockdown efficiency and the tissue specific expression patterns of circadian and chemosensory genes. A. Determination of knockdown efficiency (by qRT-PCR; 4 days after dsRNA injections) of 10 genes (per, tim, clk, cry 1, putative to1, to2, to3, obp 4, obp 22, and obp26) in antennae (that includes maxillary palps and proboscis), head, and the rest of the body of A. gambiae adult female mosquitoes. The level of the gene transcripts in each body part was normalized with AgS7. The graph shows the relative expression of each gene after knockdown, as compared to that for the GFP treated control mosquitoes (set to 1.0). N/A refers to the tissue in which the corresponding gene is not expressed. The error bars indicate the standard errors. B. Tissue specific expression (qRT-PCR products run in agarose gel) of the above mentioned 10 genes in adult female mosquitoes: the antennae (including maxillary palps and proboscis), head and the rest of the body. For all three body parts, equal amounts of RNA were taken and S7 gene of A. gambiae was the internal control. The numbers in brackets corresponds to the 10 genes in both A and B sets.