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Figure 10 | BMC Physiology

Figure 10

From: MicroCT for comparative morphology: simple staining methods allow high-contrast 3D imaging of diverse non-mineralized animal tissues

Figure 10

A neuropteran insect (genus Sisyra ) showing musculature and general soft tissue morphology. Fixed in Bouin's fluid and stained with I2E, scanned in ethanol. Virtual horizontal sections one voxel thick (top), and cutaway volume renderings (bottom). Muscles are especially clearly defined, and the complete penetration of iodine makes it more suitable than PTA or osmium for whole insect specimens. Scale bars, 100 μm. Samples from Dominique Zimmermann, Natural History Museum, Vienna. Voxel size 4.3 μm (head + thorax, left), 2.0 μm (head, right).

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BMC Physiology

ISSN: 1472-6793

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