Table 1 Real-time PCR primer pairs and assay conditions.
From: Exercise-induced up-regulation of MMP-1 and IL-8 genes in endurance horses
Gene | Accession number | Primers (forward, reverse) | Amplicon length, bp | Efficiency of assay, % | r 2 |
|---|---|---|---|---|---|
MMP-1 | NM_001081847 | CTGCTGCTGCTGCCACTC TCTCCATCACTCTTCAGGTTGTAG | 131 | 100.8 | 1.000 |
IL-8 | NM_001083951 | CTGGCTGTGGCTCTCTTG CAGTTTGGGATTGAAAGGTTTG | 132 | 97.8 | 0.999 |
HPRT | AY372182 | AATTATGGACAGGACTGAACGG ATAATCCAGCAGGTCAGCAAAG | 121 | 93.9 | 1.000 |
SDHA | DQ402987 | GAGGAATGGTCTGGAATACTG GCCTCTGCTCCATAAATCG | 91 | 96.0 | 0.999 |