Figure 4

Knockdown of AaegPTEN6 in the abdomen/fat body and ovary: Female mosquitoes (48 h post eclosion) were injected with 2 μg of dsRNA (0.5 μl of a 4 μg/μl solution). Injected mosquitoes were allowed to recover for 48 h before being mated with male mosquitoes and provided with a bloodmeal. At 48 h post-bloodmeal (96 h post-injection) we generated cDNA from the total RNA of abdomens/fat body and ovaries to assess the transcript knockdown after specific dsRNA injections. qRT-PCR was performed using primers specific to AaegPTEN6 and actin as a control. Abdomens/fat body injected with universal AaegPTEN dsRNA had a 90% reduction in AaegPTEN6 transcript, while those injected with AaegPTEN6 specific dsRNA had a 98% reduction in AaegPTEN6 transcript. Similarily, ovaries injected with universal AaegPTEN dsRNA had a 51% reduction in AaegPTEN6 transcript, while those injected with AaegPTEN6 specific dsRNA had a 99% reduction in AaegPTEN6 transcript relative to control mosquitoes injected with DsRed dsRNA. Different letters indicate a significant difference between samples.