Analysis of knockout mice suggests a role for VGF in the control of fat storage and energy expenditure

BMC Physiology

Table 5 Expression of genes in epididymal WAT and interscapular BAT that contribute to sympathetic nervous system activity in VGF knockout and wild type mice.

Tissue	Gene	Mean Cycle Threshold (C_t) Value	Std. Error	Fold Difference in mRNA Levels (2^ΔΔCt)	P-Value	RNA Levels in VGF_C57 -/- vs +/+ mice
BAT	β3AR	Vgf-/Vgf- 2.55 Vgf+/Vgf+ 2.90	.456 .461	1.3	NS
BAT	β2AR	Vgf-/Vgf- 4.50 Vgf+/Vgf+ 7.54	.730 .204	8.0	.0029	Increased
BAT	β1AR	Vgf-/Vgf- 8.41 Vgf+/Vgf+ 8.13	.222 .193	1.2	NS
WAT	β3AR	Vgf-/Vgf- 2.35 Vgf+/Vgf+ 1.37	.750 .278	1.9	NS
WAT	β2AR	Vgf-/Vgf- 5.71 Vgf+/Vgf+ 7.21	.361 .192	2.8	.0104	Increased
WAT	β1AR	Vgf-/Vgf- 4.43 Vgf+/Vgf+ 5.45	.634 .143	2.0	NS

Real time RT-PCR analysis was carried out in quadruplicate as described in the Methods. The fold difference was calculated from the indicated mean cycle threshold (C_t) values, the amplification cycle when product can first be detected: lower C_t values correspond to greater abundance. C_t values were normalized to the expression of the housekeeping gene beta-actin (ΔΔCt), and fold difference in mRNA calculated as 2^ΔΔCt, as detailed in Methods, with p ≤ 0.05 considered significant.

ISSN: 1472-6793