Figure 2

IL-1β, IL-6, hexosaminidase and lactate dehydrogenase levels. (A-B) Plasma IL-1β and IL-6 levels for WT and MK2^-/- mice, 2.5h after i.v. injection of 450β/kg TNF (n^PBS (WT and MK2^-/-)=10, n^TNF (WT)=5, n^TNF (MK2^-/-)=5). (C-D) Plasma hexosaminidase and lactate dehydrogenase plasma levels for WT and MK2^-/- mice, 2.5h after i.v. injection of 450μg/kg TNF. Pooled data from 2 separate experiments is shown (n^PBS (WT and MK2^-/-)=17, n^TNF (WT)=10, n^TNF (MK2^-/-)=8). Comparisons were made between baseline (PBS) and TNF challenged animals (#), and between genotypes (*) via one-way ANOVA with Sidak’s multiple comparisons test. Error bars indicate SD. ****, p≤0.0001; **, p≤0.01; *, p≤0.05.