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Fig. 10 | BMC Physiology

Fig. 10

From: Subcellular dissemination of prothymosin alpha at normal physiology: immunohistochemical vis-a-vis western blotting perspective

Fig. 10

Immuohistochemical analysis of PTMA in (a) mouse ileum tissue and (b) colon. Whole animal vascular perfusion fixation was conducted with PFA and the required tissues were isolated. The ileum and colon were processed for immunohistochemistry by embedding to paraffin wax. Tissue sections were stained with AntiCT (a)i and (b)i and 2 F11 (a)ii and(b)ii together with relevant fluorescent tagged antibodies. Sections were then washed, counterstained with Hoechst (a)iii and(b)iii and processed for fluorescence microscopy. The ileum and colon depict nucleic PTMA staining with AntiCT (a and b)i and cytoplasmic staining with 2 F11 (a and b)ii

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BMC Physiology

ISSN: 1472-6793

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