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Fig. 2 | BMC Physiology

Fig. 2

From: Subcellular dissemination of prothymosin alpha at normal physiology: immunohistochemical vis-a-vis western blotting perspective

Fig. 2

Determination of cross reactivity of the antibodies with rat and human PTMA. GST tagged recombinant mouse, rat and human PTMA were loaded onto gel for electrophoresis and blotted on to nitrocellulose membranes thereafter. Blotted membranes were incubated with supernatant from the hybridoma cultures from different clones as indicated. Antibodies from most clones showed crossreactivity with rat and human PTMA. Some showed relatively similar or different signal intensities indicative of the affinity of the antibodies to the particular antigens. Clones 9, 11 and 12 lost their ability to secret antibodies

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BMC Physiology

ISSN: 1472-6793

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