Table 1 Primer and probe sequences for quantitative real-time RT-PCR
Gene Symbol | Gene Full name | Method | Primer | Probe (5’-FAM, 3’-TAMRA) |
|---|---|---|---|---|
pparg | peroxisome proliferator-activated receptor gamma | TAQMAN | F 5‘-GCTGCACAGGCGCTTCA R 5‘-CTCCAGCTCCTCCAGTTCCA | CAGAAAGCTTCACTCTCCGCTGATATGGTG |
fabp11a | fatty acid binding protein 11a | TAQMAN | F 5‘-GGTTGACAAATTCGTAGGAACGT R 5‘-AACCCACACCTATAGCCTTCATG | AATGACCACCAGCGACAACTTTGACGA |
fasn | fatty acid synthase | TAQMAN | F 5‘-ACACGGTTCACGCATTTGTG R 5‘-GACCCATCTTCCGTAGCATATCA | AGCTATTCAGGTTGCCCAGA |
srebf1 | sterol regulatory element binding transcription factor 1 | TAQMAN | Predesigned (Dr03093012_m1; Thermo Fisher) | |
atgl | adipose triglyceride lipase | SYBR Green | F 5‘-GCGTGACGGATGGAGAAA R 5‘-AGGCCACAGTAAACAGGAATAT | |
hsl | hormone-sensitive lipase | SYBR Green | F 5‘-CGGCAAGGACAGGACAGT R 5‘-GCATGGAGAAAGAGGAGCT | |
lpl | lipoprotein lipase | TAQMAN | F 5‘-CTGAGGGCTCTCGTTCATAAAGA R 5‘-AATCCATCAAAGACTGTAACTTCAATACA | CTCTCAAACATACCCGTGACCGTCCATC |
il1b | interleukin 1 beta | TAQMAN | F 5‘- TCATCGCCCTGAACAGAATG R 5‘-TCACTTCACGCTCTTGGATGAC | AGCACATCAAACCCCAATCCACAGAGTT |
tnfa | tumor necrosis factor alpha | TAQMAN | Predesigned (Dr03126848_g1; Thermo Fisher) | |
col1a1a | collagen, type I, alpha 1a | SYBR Green | F 5‘-GCTTTTGGCAAGAGGACAAG R 5‘-TGTCTTCGCAGATCACTTCG | |
bactin2 | beta-actin 2 | TAQMAN | F 5‘-TCCCCTTGTTCACAATAACCTACTAA R 5‘-CATACCGGAGCCGTTGTCA | AGCGATTTCCTCATCCATGGCTGTGT |
tbp | TATA-box binding protein | TAQMAN | F 5‘-CCTGCGAATTATCGTTTACGTCTT R 5‘-ACGGCATCATAGGACTGAAAATG | TTGCTTCATAACCTGTCAGCATGGAGCA |