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Fig. 1 | BMC Physiology

Fig. 1

From: Ryanodine-induced vasoconstriction of the gerbil spiral modiolar artery depends on the Ca2+ sensitivity but not on Ca2+ sparks or BK channels

Fig. 1

Ca2+ sparks in smooth muscle cells of the pressurized spiral modiolar artery. a Ca2+ spark site captured during a 5 s frame scan of SMCs in a pressurized (60 cmH2O) SMA. Grey panel (top panels) depicts the average of 10 frames that do not contain a Ca2+ spark. Outline (in red) depicts the visible portion of the cell. Scale bar = 2 μm. Pseudo-color image depicts an average (left middle panel) and a large (right middle panel) Ca2+ spark at its peak. Regions of interest (ROIs) are 2 μm × 2 μm. (Bottom panels) show a 3D rendering of the 2D images. Traces (green and red) represent the fluorescence intensity changes occurring at the corresponding ROIs shown in the panels above. b Histogram showing the distribution of the calculated spatial area of Ca2+ spark sites. c Contrast-adjusted 5 s line-scan recording of a Ca2+ spark site in a SMC of a pressurized (60 cmH2O) SMA and the corresponding fluorescence intensity traces recorded in PSS and in the presence of 10 μM ryanodine (Ryn). d Spark frequency in PSS (n = 9), 100 μM tetracaine (Tet, n = 3) and 10 μM ryanodine (Ryn, n = 4)

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BMC Physiology

ISSN: 1472-6793

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