- Research article
- Open Access
Sodium selectivity of Reissner's membrane epithelial cells
© Yamazaki et al; licensee BioMed Central Ltd. 2011
Received: 22 October 2010
Accepted: 1 February 2011
Published: 1 February 2011
Sodium absorption by Reissner's membrane is thought to contribute to the homeostasis of the volume of cochlear endolymph. It was previously shown that the absorptive transepithelial current was blocked by amiloride and benzamil. The most commonly-observed target of these drugs is the epithelial sodium channel (ENaC), which is composed of the three subunits α-,β- and γ-ENaC. However, other less-selective cation channels have also been observed to be sensitive to benzamil and amiloride. The aim of this study was to determine whether Reissner's membrane epithelial cells could support parasensory K+ absorption via amiloride- and benzamil-sensitive electrogenic pathways.
We determined the molecular and functional expression of candidate cation channels with gene array (GEO GSE6196), RT-PCR, and whole-cell patch clamp. Transcript expression analysis of Reissner's membrane detected no amiloride-sensitive acid-sensing ion channels (ASIC1a, ASIC2a, ASIC2b) nor amiloride-sensitive cyclic-nucleotide gated channels (CNGA1, CNGA2, CNGA4, CNGB3). By contrast, α-,β- and γ-ENaC were all previously reported as present in Reissner's membrane. The selectivity of the benzamil-sensitive cation currents was observed in whole-cell patch clamp recordings under Cl--free conditions where cations were the only permeant species. The currents were carried by Na+ but not K+, and the permeability of Li+ was greater than that of Na+ in Reissner's membrane. Complete replacement of bath Na+ with the inpermeable cation NMDG+ led to the same inward current as with benzamil in a Na+ bath.
These results are consistent with the amiloride/benzamil-sensitive absorptive flux of Reissner's membrane mediated by a highly Na+-selective channel that has several key characteristics in common with αβγ-ENaC. The amiloride-sensitive pathway therefore absorbs only Na+ in this epithelium and does not provide a parasensory K+ efflux route from scala media.
The inner ear has absorptive pathways for both Na+ and K+ that contribute to the homeostasis of the composition of endolymph, the luminal fluid. The regulation of the ion composition of endolymph is essential for normal hearing [1, 2]. Transepithelial K+ efflux through the sensory hair cells in the cochlea is responsible for detection of sound. Parasensory K+ absorption through other cell types is needed to compensate for changes in sensory cell K+ flux due to changes in levels of stimulation from acoustic inputs. The cochlear outer sulcus is an epithelial domain known to participate in absorption of both K+ and Na+.
Absorptive mechanisms are needed to remove Na+ from endolymph in order to maintain osmotic balance, to prevent loading of sensory hair cells with Na+ and to maintain functional physical properties of the tectorial membrane. Na+, like K+, is absorbed via nonselective cation channels in the apical membranes of outer sulcus cells. In addition, Na+ appears to be absorbed via an amiloride-sensitive pathway in Reissner's membrane (RM) of the cochlea.
The transepithelial current across RM was shown to be inhibited by amiloride and its analog, benzamil [4, 5]. The most commonly-observed target of these drugs is the epithelial sodium channel (ENaC), which is usually composed of the three subunits α-,β- and γ-ENaC. However, other combinations of ENaC subunits and other cation channels have also been observed to be sensitive to amiloride and benzamil. Further, those channels are not as selectively permeable to Na+ over K+ and would therefore provide a potential pathway for parasensory K+-absorption.
In view of the high luminal concentration of K+ in the inner ear (ca. 150 mM) and the importance of K+ efflux pathways for endolymph homeostasis, we investigated whether RM epithelial cells could support parasensory K+ absorption via amiloride-sensitive electrogenic pathways. The results show that acutely isolated RM has a highly Na+-selective transport pathway, without detectable contributions from K+. The processes studied have several properties of the classical ENaC channel including inhibition by amiloride and benzamil, high selectivity for Na+ over K+ and a higher permeability to Li+ over Na+.
We have shown in previous studies that Reissner's membrane in mouse and gerbil absorbs Na+ from the cochlear lumen by electrogenic transepithelial transport, which was apparently mediated by apical ENaC, basolateral Na+,K+-ATPase, and basolateral K+ channels [4, 5]. This Na+ absorption was blocked by amiloride and benzamil. The most commonly-observed target of these drugs is ENaC, comprised of the three subunits α-, β- and γ-ENaC. We addressed the question of cation selectivity of this pathway in Reissner's membrane epithelial cells with 5 series of patch clamp experiments and selective candidate gene expression analysis.
Benzamil-sensitive currents under whole-cell patch clamp
Inward and outward wholecell patch clamp currents, conductances and reversal voltage under established cationic conditions.
Na or K Bath
Na - Li
All cations - benzamil
Indeed, benzamil (1 μM) reduced the inward current when the KCl-rich pipette solution (P1) mimicked the presumed intracellular composition (although Cl- was higher than often observed) and when the NaCl-rich bath (B1) mimicked the basolateral (perilymph) composition, (Additional file 1: Fig. S1 and Fig. S3; Table 1). Benzamil was used throughout these experiments at a concentration (1 μM) that yielded a complete inhibition of the transepithelial current  and significantly inhibited the inward whole-cell current (at -100 mV; I-100) by 48.5%; from -1305 ± 277 pA to -671 ± 146 pA (n = 7). These whole-cell data are consistent with the transepithelial measurements of Na+ absorption by Reissner's membrane.
Expression of benzamil-sensitive cation channels in RM
Transcript analysis of amiloride-sensitive channel genes in Reissner's membrane.
GenBank Accession No.
Call Gene array*
Na+ and K+ conductance of benzamil-sensitive current
Bath and pipette solutions (mM)
In symmetrical K+-rich bath and pipette solutions (B3, P3), the current and conductance at -100 mV were significantly smaller than in Na+ (Figure 1, 2; Table 1). Benzamil had no significant effect on the inward whole-cell current (carried mostly by bath K+ at -100 mV) (mean decreased insignificantly by 9.8%; from I-100 = -476 ± 112 pA to -429 ± 130 pA, n = 8). A representative experiment is shown in Figure 1 and a summary of similar experiments is shown in Figure 2 and Table 1.
Li+/Na+ permeability ratio of benzamil-sensitive current
It was to be expected that there would be no change in the observed Vr between the presence and absence of benzamil since the reversal potential for both Na+-selectective and NSC channels are both = 0. By contrast, if Li+ is more permeable than Na+, Vr would be expected to become more positive (see second series, below).
where ΔVr is the change in reversal potential, R the universal gas constant, T the absolute temperature, z the valence, F the Faraday constant, [Li]o the extracellular Li+ concentration, [Na]o the extracellular Na+ concentration and PLi and PNa the permeabilities of the membrane for Li+ and Na+. The shift of Vr by 7.0 mV in the current study corresponds to a PLi/PNa ratio of 1.3. This result is within the range of reports by others for the ratio in αβγ-ENaC (1.3 [13, 14], 1.6 , 1.8 ).
Benzamil-insensitive cation channels
We hypothesized that the residual current after inhibition by benzamil could be transported through nonselective cation channels, although a substantial contribution of NSC channels was unlikely since K+ conductance was relatively small. Alternatively, the residual current could be a patch leakage current.
It was first observed that transcripts of TRPV4, an amiloride-insensitive NSC channel, were present by both gene array and RT-PCR (Table 2). The amiloride-insensitive channels HCN1-4 are permeable to K+ and Na+ (PK/PNa ~4 ) and were found to be absent by RT-PCR, although some false positives in the gene array were found (Table 2).
We tested for the presence of benzamil-insensitive currents by comparing the inward current in the presence and absence of benzamil with a substitution of the impermeable cation NMDG+ for Na+ in the bath.
Na+ in the pipette was reduced to 15 mM (P4) in order to confirm the Na+-selectivity of the current in this experimental series. The large decrease in Vr between Na+ (B2) and NMDG+ (B5) (from 29.8 ± 1.0 mV to -27.8 ± 3.6 mV, n = 5; Figure 5 and Table 1) is consistent with a large Na+ conductance. The small, but significant, decrease in Vr between ± benzamil could conceivably be due to the presence of an H+ conductance and the small pH gradient between intra- and extracellular solutions. The equilibrium potential for Na+ was +60 mV and for H+ was +6 mV. The absence of any significant difference (Table 1) in I+100 (14834 ± 342 pA vs 1666 ± 243 pA, n = 5) in NMDG+ and Na+ baths (B5 and B2) is consistent with the current at +100 mV being carried predominantly by the efflux of intracellular Na+, since the pipette concentration (P4) remained the same for both bath solutions.
Active Na+ absorption is needed to remove Na+ that has entered cochlear endolymph through as-yet-undefined pathways. Elevated Na+ would provide an osmotic driving force for water entry that would lead to endolymphatic hydrops, a pathologic swelling of the luminal space. In addition, the cochlear epithelium requires parasensory K+-efflux pathways to compensate for changes in K+ efflux through sensory hair cells during changes in auditory stimulation.
In addition to Na+ and K+ absorption from endolymph via the outer sulcus cells in the cochlear lateral wall , Reissner's membrane epithelium has been found to actively absorb Na+ from endolymph via amiloride- and benzamil-sensitive pathways. This transport was thought to be mediated by the epithelial Na+ channel (ENaC) in the apical membrane of these cells [4, 5].
The classical ENaC is highly selective to the passage of Na+ over K+ (PNa/PK > 80). However, there are reports of poorly-selective epithelial Na+ absorptive pathways under some culture conditions and this can be due to either altered ENaC subunit stoichiometry or increased expression of unrelated nonselective cation channels . Heteromeric channels composed of the α-, β- and γ-ENaC subunits are highly Na+-selective, while homomeric α-ENaC channels are also permeable to K+. Homo- and hetero-meric ENaC are pharmacologically defined by their inhibition by amiloride and its analogs . All 3 ENaC subunits are expressed in Reissner's membrane , but the stoichiometric subunit association in the apical membrane of Reissner's membrane epithelial cells is not known. In addition, other cation channels such as acid sensitive ion channels (ASIC) and cyclic nucleotide gated channels (CNG) have been observed to be sensitive to amiloride and benzamil (micromolar to submicromolar range) [20, 21].
A vestige of doubt therefore remained whether the benzamil-sensitive current could also carry K+ as well as Na+.
We tested the hypothesis that the benzamil-sensitive Na+ absorption pathway in Reissner's membrane could also carry a K+ current. Our results showed the absence of transcript expression of many candidate nonselective cation channels and the functional absence of both benzamil-sensitive and -insensitve nonselective cation currents. The results demonstrated a high selectivity of the benzamil-sensitive pathway to Na+ over K+.
One further test of the molecular identity of the benzamil-sensitive pathway was a comparison of the permeability to Li+ and Na+. The greater permeability to Li+ is consistent with the benzamil-sensitive current carried by the classical αβγ -ENaC.
Tissue preparation for electrophysiological recordings
Tissue was obtained, prepared and analyzed as described previously . Mice (C57BL/6) were anesthetized with 4% tribromoethanol (0.014 ml/g body wt ip) and killed following a protocol approved by the Institutional Animal Care and Use Committee of Kansas State University. The lateral wall of the cochlear apical turn was isolated from the temporal bone, the stria vascularis removed and Reissner's membrane was gently pushed into the same plane as the spiral ligament. Reissner's membrane consists of an epithelial cell layer facing the endolymph and a mesothelial cell layer facing the perilymph, the two layers being separated by a basement membrane. The tissue was folded with the apical membrane of the epithelium on the exterior of the loop in order to form patch clamp seals with the apical membrane (Additional file 1: Fig. S3).
Whole cell patch clamp recording
Currents were recorded using the whole-cell configuration of the patch clamp technique, similar to our previous study . Patch pipettes were made from borosilicate glass capillaries (1B150F; World Precision Instruments, Sarasota, FL), pulled in three stages with an electrode puller (Narishige, East Meadow, NY). Inner diameter of the tip was approximately 2 μm and after heat polishing the pipettes had resistances of 3.3 - 9.6 MΩ (n = 31) in the different bath solutions.
Currents were recorded with an Axopatch 200A amplifier (Axon Instruments, Foster City, CA) and low-pass filtered at 1 kHz. Current signals were digitized at 5 kHz using a computer with a Digidata 1322A (Axon Instruments) and pCLAMP 9 software (clampex9, Axon Instruments) was used for voltage step protocol recordings. In addition, AxoScope software (Axon Instruments) with MiniDigi 1A (Axon Instruments) data acquisition hardware was simultaneously used for continuous trace recordings and current signals were digitized at 1 kHz. The temperature was maintained at 37°C on a glass-bottomed bath chamber by a continuous, warmed perfusion and supplemental chamber heater.
For the pipette solution containing high K+ (P3) and bath containing high Na+ (B2), the data were corrected for the liquid junction potential of 4.1 mV. For the pipette solution containing low Na+ and high NMDG+ (P4) and baths containing high Na+ (B2) and high NMDG+ (B5), the data were corrected for the liquid junction potentials of -6.9 mV and -6.2 mV (the latter includes the liquid junction potential change of 0.7 mV at the reference electrode). Liquid junction potentials in symmetric Na-Methanesulfonate, K-Methanesulfonate, NMDG-Methanesulfonate were near zero, since voltage offset was adjusted when the pipette was immersed in each bath solution. The Li+ data were corrected by adding the 0.3 mV junction potential change at the reference electrode.
Two voltage protocols were used. Protocol 1: A series of pulses were applied for 300 ms each from a holding potential of 0 mV to steps from +100 to -100 mV in 20 mV decrements. The protocol was repeated every 16.7 s. Current-voltage (I-V) relationships were determined from averaging 30 ms of steady-state currents near the end of each voltage step. Protocol 2: This series only differed from the first protocol by the step duration (200 mS) and the repetition rate (5.3 s). The I-V relationships were obtained from these current averages plotted against the command voltage, corrected for the liquid junction potential and an estimate of the voltage drop across the pipette resistance. These corrected curves were used to obtain the currents and conductances at ±100 mV (I-100, I+100, g+100 and g-100) and the reversal voltage, Vr (Additional file 1: Fig. S4). Data were plotted with Origin software, version 7 (OriginLab Software, Northampton, MA).
Solutions and chemicals
Table 3 shows the composition of the solutions of pipette and bath for electrophysiological recordings. The osmolarity of these solutions was about 311-318 mOsm. Benzamil (Sigma #B-2417) was predissolved (1 mM) in dimethylsulfoxide (DMSO), and was used at a final concentration of 1 μM benzamil and 0.1% DMSO. Pipette solutions (P1, P2, P3, P4) were buffered to 100 nM free Ca2+. The KCl-rich pipette solution (P1) was adjusted to pH 7.3 at room temperature, which corresponds to about 7.2 at 37°C and alters the Ca2+/EGTA ratio at that elevated temperature. Pipettes using P2, P3 and P4 were backfilled with 15 mM Cl- substituted for methanesulfonate in order to make stable contact with the Ag/AgCl electrode. The backfill solutions contained the dye fast green to visualize the interface with the tip solution, which filled the first 10 mm of the pipette. All pipette solutions for patch clamp were passed through 0.22 μm cellulose acetate filters (Corning).
RNA isolation for RT-PCR
Four mice (C57BL/6, 4-10 weeks old) were anesthetized with 4% tribromoethanol (0.014 ml/g body wt ip) and killed for each collection of total RNA. The two temporal bones were removed from each mouse after a transcardial perfusion of a phosphate-buffered saline (PBS) solution to reduce both cell swelling and contamination from blood cells. Reissner's membrane was dissected in PBS, which was changed twice during isolation of Reissner's membrane to reduce cross contamination from other tissues. All procedures were approved by the Institutional Animal Care and Use Committee of Kansas State University. Total RNA was isolated from the tissue using an RNeasy Micro Kit with carrier RNA (Qiagen, Valencia, CA). RNA used as positive control was from mouse kidney, mouse brain (Ambion, Austin, TX). The quality and quantity of RNA were determined with a Bioanalyzer (Additional file 1: Fig. S3B; Agilent, Palo Alto, CA). RT-PCR was conducted as described before .
Data were expressed as the mean ± S.E.M. (n = number of whole cell patches). Increases and decreases in current and conductance were determined by Student's paired or unpaired t-test as appropriate for the specific data sets. Differences were considered statistically significant at a level of P < 0.05 (Microsoft Excel).
MY is a clinician/researcher in the Dept. of Otolaryngology-Head and Neck Surgery at Tohoku University who conducted this research in partial fulfillment of requirements for the PhD degree at the Tohoku University Graduate School of Medicine. KXK contributed to this work while a candidate for a Masters degree at Kansas State University and upon completion of the requirements for the Master of Science degree, she has become a doctoral candidate in the Neuroscience Training Program at the University of Wisconsin. DCM is a University Distinguished Professor at Kansas State University in the Dept. of Anatomy & Physiology and heads the Cellular Biophysics Laboratory.
This work was supported by NIH grants R01-DC00212 and P20-RR017686 to DCM. We thank Dr. Philine Wangemann for helpful discussions.
- Marcus DC, Wangemann P: Inner ear fluid homeostasis. The Oxford Handbook of Auditory Science: The Ear. Edited by: Fuchs PA. 2010, Oxford: Oxford University Press, 213-230.Google Scholar
- Marcus DC, Wangemann P: Cochlear and Vestibular Function and Dysfunction. Physiology and Pathology of Chloride Transporters and Channels in the Nervous System--From molecules to diseases. Edited by: Alvarez-Leefmans FJ, Delpire E. 2009, New York: Elsevier, 425-437.Google Scholar
- Chiba T, Marcus DC: Nonselective cation and BK channels in apical membrane of outer sulcus epithelial cells. J Membr Biol. 2000, 174: 167-179. 10.1007/s002320001041.PubMedView ArticleGoogle Scholar
- Lee JH, Marcus DC: Endolymphatic sodium homeostasis by Reissner's membrane. Neuroscience. 2003, 119: 3-8. 10.1016/S0306-4522(03)00104-0.PubMedView ArticleGoogle Scholar
- Kim SH, Kim KX, Raveendran NN, Wu T, Pondugula SR, Marcus DC: Regulation of ENaC-mediated sodium transport by glucocorticoids in Reissner's membrane epithelium. Am J Physiol, Cell Physiol. 2009, 296: C544-C557. 10.1152/ajpcell.00338.2008.PubMedPubMed CentralView ArticleGoogle Scholar
- Marcus DC, Raveendran NN, Wu T: Reissner's membrane, mouse, GEO GSE6196. [http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE6196]
- Yagi J, Wenk HN, Naves LA, McCleskey EW: Sustained currents through ASIC3 ion channels at the modest pH changes that occur during myocardial ischemia. Circ Res. 2006, 99: 501-509. 10.1161/01.RES.0000238388.79295.4c.PubMedView ArticleGoogle Scholar
- Ishikawa T, Marunaka Y, Rotin D: Electrophysiological characterization of the rat epithelial Na+ channel (rENAC) expressed in MDCK cells. Effects of Na+ and Ca2+. J Gen Physiol. 1998, 111: 825-846. 10.1085/jgp.111.6.825.PubMedPubMed CentralView ArticleGoogle Scholar
- Warncke J, Lindemann B: Voltage dependence of Na channel blockage by amiloride: relaxation effects in admittance spectra. J Membr Biol. 1985, 86: 255-265. 10.1007/BF01870605.PubMedView ArticleGoogle Scholar
- Palmer LG: Modulation of apical Na permeability of the toad urinary bladder by intracellular Na, Ca, and H. J Membr Biol. 1985, 83: 57-69. 10.1007/BF01868738.PubMedView ArticleGoogle Scholar
- Bize V, Horisberger JD: Sodium self-inhibition of human epithelial sodium channel: selectivity and affinity of the extracellular sodium sensing site. Am J Physiol Renal Physiol. 2007, 293: F1137-F1146. 10.1152/ajprenal.00100.2007.PubMedView ArticleGoogle Scholar
- Hille B: Selective Permeability: Independence. Ion Channels of Excitable Membranes. 2001, Sunderland, MA: Sinauer Associates, Inc, 441-470. ThirdGoogle Scholar
- del Monaco S, Assef Y, Kotsias BA: Epithelial sodium channel in a human trophoblast cell line (BeWo). J Membr Biol. 2008, 223: 127-139. 10.1007/s00232-008-9119-3.PubMedView ArticleGoogle Scholar
- Snyder PM, Olson DR, Bucher DB: A pore segment in DEG/ENaC Na(+) channels. J Biol Chem. 1999, 274: 28484-28490. 10.1074/jbc.274.40.28484.PubMedView ArticleGoogle Scholar
- Ji HL, Bishop LR, Anderson SJ, Fuller CM, Benos DJ: The role of Pre-H2 domains of alpha- and delta-epithelial Na+ channels in ion permeation, conductance, and amiloride sensitivity. J Biol Chem. 2004, 279: 8428-8440. 10.1074/jbc.M312012200.PubMedView ArticleGoogle Scholar
- Lyashchenko AK, Tibbs GR: Ion binding in the open HCN pacemaker channel pore: fast mechanisms to shape "slow" channels. J Gen Physiol. 2008, 131: 227-243. 10.1085/jgp.200709868.PubMedPubMed CentralView ArticleGoogle Scholar
- Marcus DC, Chiba T: K+ and Na+ absorption by outer sulcus epithelial cells. Hear Res. 1999, 134: 48-56. 10.1016/S0378-5955(99)00074-X.PubMedView ArticleGoogle Scholar
- Matalon S, Lazrak A, Jain L, Eaton DC: Invited review: biophysical properties of sodium channels in lung alveolar epithelial cells. J Appl Physiol. 2002, 93: 1852-1859.PubMedView ArticleGoogle Scholar
- Alvarez de la Rosa D, Canessa CM, Fyfe GK, Zhang P: Structure and regulation of amiloride-sensitive sodium channels. Annu Rev Physiol. 2000, 62: 573-594. 10.1146/annurev.physiol.62.1.573.PubMedView ArticleGoogle Scholar
- Vandorpe DH, Ciampolillo F, Green RB, Stanton BA: Cyclic nucleotide-gated cation channels mediate sodium absorption by IMCD (mIMCD-K2) cells. Am J Physiol, Cell Physiol. 1997, 272: C901-C910.Google Scholar
- Meltzer RH, Kapoor N, Qadri YJ, Anderson SJ, Fuller CM, Benos DJ: Heteromeric assembly of acid-sensitive ion channel and epithelial sodium channel subunits. J Biol Chem. 2007, 282: 25548-25559. 10.1074/jbc.M703825200.PubMedView ArticleGoogle Scholar
- Kim KX, Marcus DC: Inward-rectifier chloride currents in Reissner's membrane epithelial cells. Biochem Biophys Res Commun. 2010, 394: 434-438. 10.1016/j.bbrc.2010.03.048.PubMedPubMed CentralView ArticleGoogle Scholar
- Schoenmakers TJ, Visser GJ, Flik G, Theuvenet AP: CHELATOR: an improved method for computing metal ion concentrations in physiological solutions. BioTechniques. 1992, 12: 870-879.PubMedGoogle Scholar
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